Agilent 1260 Infinity ll LC System w/ Variable Wavelength and Refractive Index Detectors Unit 2 in Walpole, MA, USA

- Made in 2021
- Good cosmetic condition
- Technical sales consultation available
- CONTINENTAL US 120 DAYS WARRANTY & 60 DAYS INTERNATIONAL
---------------------------------------------------------------

Here is what you will receive:

- Agilent 1260 Infinity II Quaternary Pump p/n: G7111B
- Agilent 1260 Infinity II Vialsampler p/n: G7129A with 900mkL sample loop
- Agilent InfinityLab Sample Thermostat p/n: G7167-60101
- Agilent 1260 Infinity II Multicolumn Thermostat p/n: G7116A with 2 position/6 port switching valve p/n 5067-4282
- Agilent 1260 Infinity II VWD Detector p/n: G7114A with standard cell 4MPa (40bar) G1314-60186
- Agilent 1260 Infinity II RID Detector p/n: G7162A
- Agilent 1200 Instant Pilot G4208A
- Solvent bottle tray
- Power and communication cables

Please note: The computer used for testing and demonstration is not included. Computers and software will need to be purchased separately.

Agilent 1260 Infinity II LC System Configuration:

- Detector modules: Variable Wavelength Detector (VWD), Refractive Index Detector (RID)
- Pump type: dual-piston in series, servo-controlled variable-stroke quaternary pump
- Flow range:0.001-10 mL/min (recommended 0.2–10 mL/min)
- Injection range: 0.1-900 μL in 0.1 μL increments; Pressure limit: 40 MPa (400 bar)
- Sample capacity: 100 x 2 mL vials
- Maximum Number of Temperature Zones: 2 (Multicolumn Thermostat)
- System pressure operating range: up to 60 MPa (600 bar) to 5 mL/min; 20 MPa to 10 mL/min

Agilent 1260 Infinity II Quaternary Pump:

- Hydraulic system: dual piston in series pump with servo-controlled variable stroke drive, power transmission by gears and ball screws, floating pistons
- Settable flow range: set points 0.001-10 mL/min, in 0.001 mL/min increments
- Recommended flow range: 0.2-10.0 mL/min
- Flow precision: ≤0.07 % RSD, or ≤0.02 min SD, whichever is greater, based on retention time at constant room temperature
- Flow accuracy: ± 1 % or 10 μL/min, whichever is greater, pumping degassed H2O at 10 MPa (100 bar)
- Pressure operating range:

- Operating range up to 60 MPa (600 bar, 8700 psi) up to 5 mL/min

- Operating range up to 20 MPa (200 bar, 2950 psi) up to 10 mL/min

- Pressure pulsation: < 2 % amplitude (typically < 1.0 %), or < 0.3 MPa (3 bar, 44 psi), whichever is greater, at 1 mL/min isopropanol, at all pressures > 1 MPa (10 bar, 145 psi)
- Compressibility compensation: user-selectable, based on mobile phase compressibility
- Recommended pH range: 1.0-12.5; solvents with pH < 2.3 should not contain acids that attack stainless steel
- Gradient formation: low-pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve
- Delay volume: 600-900 μL, dependent on back pressure; measured with water at 1 mL/min (water/caffeine tracer)
- Settable composition range: 0-100 % in 0.1 % increments
- Composition precision: < 0.2 % RSD or < 0.04 min SD, whichever is greater, at 1 mL/min; based on retention time at constant room temperature
- Degassing unit number of channels: 4
- Internal volume per channel: 1.5 mL
- Weight 17.6 kg (38.8 lbs)
- Dimensions (H×W×D): 18 x 39.6 x 43.6 mm (7.1x15.6x17.2 in.)
- Power requirements: 100-240 V~, ± 10 % 50/60 Hz, ± 5 % 80 VA, 65 W

Agilent 1260 Infinity II Vialsampler:

- Injection range: 0.1-900 μL in 0.1 μL increments with 900 μL up to 40 MPa
- Injection precision: <0.25 % RSD of peak areas from 5 μL to 100 μL
- Pressure range: 0-40 MPa (0 - 400 bar, 0 - 5801 psi)
- Sample viscosity range: 0.2 - 5.0 cP
- Sample capacity: 100 x 2 mL vial
- Carryover: <0.004 % (40 ppm) with needle wash
- Injection cycle time: 18s. for draw speed 200 μL/min; Ejection speed: 200 μL/min; Injection volume: 1 μL
- Minimum sample volume: 1 μL from 5 μL sample in a 100 μL microvial, or 1 μL from a 10 μL sample in a 300 μL microvial
- Weight: 19 kg (41.9 lbs)
- Dimensions (H×W×D): 32 x 39.6 x 46.8 cm. (12.8 x 15.6 x 18.4 in.)
- Power requirements: 100-240 V~, ± 10 % 50/60 Hz, ± 5 % 350 VA, 350 W, 1195 BTU/h

Agilent 1260 Infinity II Sample Thermostat:

- Operating principle: high-performance, low-energy consumption micro-compressor-based cooler with natural R600a coolant (Isobutane max. 0.030 kg)
- Temperature range: from 4 DegC to 40 DegC
- Temperature settable: from 4 DegC to 40 DegC in 1 DegC increments
- Temperature accuracy: 2 DegC to 6 DegC at a setpoint of 4 DegC

Agilent 1260 Infinity II Multicolumn Thermostat:

- Operating principle: thermostatted column compartment with dual, independent Peltier elements. Solvent pre-heating and still-air operation for reduction of chromatographic band-broadening under μLC conditions.
- Temperature range: 10 DegC below ambient (minimum 4 DegC) to 85 DegC
- Temperature range increment: settable in steps of 0.1 DegC
- Temperature stability ±0.1 DegC
- Temperature accuracy ±0.5 DegC (with calibration at 40 DegC)
- Temperature precision 0.05 DegC
- Independent temperature zones: 2 in a single device
- Heat-up/cool-down time: 5 min from ambient to 40 DegC; 10 min from 40 DegC to 20 DegC; 25 min from 25 DegC to 85 DegC
- Column capacity: 4 columns of up to 300 mm length plus InfinityLab Quick Connect fittings or pre-column.
- Valve head: 2 position/6 port switching valve 800 bar
- Typical application: any two-way switching, e.g., between two detectors, btw waste and detector, btw two columns
- Liquid contacts: PEEK, Stainless steel
- pH range: 0-14
- Weight: 12.5 kg (27.6 lbs)
- Dimensions: (H×W×D): 16 x 43.5 x 43.6 cm. (6.3 x 17.1 x 17.2 in.)
- Power requirements: 100-240 V~, ± 10 % 50/60 Hz, ± 5 %, 150 VA, 150 W

Agilent 1260 Infinity II Variable Wavelength Detector:

- Detection type: double-beam photometer
- Light source: deuterium lamp
- Number of signals: single and dual wavelength detection
- Maximum data rate: 120 Hz (single wavelength detection), 2.5 Hz (dual wavelength detection)
- Short-term signal noise (ASTM): <±0.25·10-5 AU, at 230 nm (single wavelength detection); <±0.80·10-5 AU, at 230 nm and 254 nm (dual wavelength detection)
- Drift: <1·10-4 AU/h, at 230 nm
- Linear absorbance range: >2.5 AU upper limit
- Wavelength range: 190 - 600 nm
- Wavelength accuracy: ±1 nm, self-calibration with deuterium lines, verification with holmium oxide filter
- Wavelength precision: <±0.1 nm
- Slit width: 6.5 nm, typical over the whole wavelength range
- Time programmable: wavelength, polarity, peak width, lamp on/off
- Flow cell: standard: 14 μL volume, 10 mm cell path length, and 40 bar (580 psi) pressure maximum
- Weight: 11 kg (24.3 lbs)
- Dimensions (H×W×D): 14 x 39.6 x 43.6 cm (5.5 x 15.6 x 17.2 in.)
- Power requirements: 100 - 240 V ± 10 %, 50/60 Hz ± 5 %, 80 VA, 70 W

Agilent 1260 Infinity II Refractive Index Detector:

- Detection type: refractive Index
- Refractive index range: 1.00-1.75 RIU, calibrated
- Measurement range: ±600·10-6 RIU
- Optical zeroing: digitally via software, manually via a set screw
- Optics temperature control: 5 DegC above ambient to 55 DegC
- Sample cell volume: 8 μL, maximum pressure: 5 bar (0.5 MPa), maximum flow rate: 5 mL/min
- Valves: Automatic purge and automatic solvent recycle
- Volumes: Inlet port to sample cell 62 μL, inlet port to outlet port 590 μL
- pH range 2.3-9.5
- Short term noise: <±1.25·10-9 RIU
- Drift: <200·10-9 RIU/hr
- Time programmable parameters: polarity, peak width
- Maximum data rate: 74 Hz
- Weight 14.7 kg (32.4 lbs)
- Dimensions (H×W×D) 18 x 39.6 x 43.6 cm (7.1 x 15.6 x 17.2 in.)
- Power requirements: 100-240 V ± 10 %, 50/60 Hz ± 5 %, 80 VA/ 70 W

Customization/Options:

- Other detectors and flow cell options might be available. Please inquire with our sales team.

Testing performed by our in-house service team: (Test Reports available upon request)

The Agilent 1260 Infinity II LC System passed all tests and was found to be fully functional:

Quaternary Pump G7111B:

- We performed the initialization test.
- We completed the software communication test.
- We tested the solvent selection valve.
- We conducted the system leak test.
- We checked the degasser.
- We tested the pressure limiter.
- We verified the flow rate accuracy.
- We assessed the solvent delivery stability.
- We conducted the leak sensor test.

Vialsampler G7129A:

- We performed the initialization test.
- We completed the software communication test.
- We verified the sample cooler set temperature accuracy.
- We conducted the sampler leak test.
- We tested the reproducibility.
- We tested injection volume accuracy.
- We conducted the leak sensor test.

Multicolumn Thermostat G7116A:

- We performed the initialization test.
- We completed the software communication test.
- We assessed the cooling and heating performance.
- We verified the column oven temperature accuracy.
- We conducted the leak sensor test.
- We verified the switching valve operation.

VWD Detector G7114A:

- We performed the initialization test.
- We completed the software communication test.
- We performed a Noise test.
- We performed the Holmium Oxide test.
- We checked wavelength accuracy.
- We verified lamp intensity.
- We conducted the leak sensor test.

RID Detector G7162A:

- We performed the initialization test.
- We completed the software communication test.
- We performed a Noise test.
- We conducted the leak sensor test.

Instrument Overview:

- The Agilent 1260 Infinity II LC System with VWD and RID provides reliable, stable chromatography performance for routine analysis.
- Built with a 1260 Infinity II Quaternary Pump to ensure precise solvent delivery and flexible gradient operation.
- Includes a vial sampler and a sample thermostat for controlled sample handling.
- Integrated multicolumn thermostat and 2-position/6-port valve support column switching and method flexibility.
- Features the 1260 Infinity II VWD and RID detectors, enabling dependable optical detection across common HPLC workflows.
- Usability is enhanced by the Agilent 1200 Instant Pilot, which provides convenient front-panel system control.
- The system emphasizes reproducibility, low drift, and long-term operation, making it suitable for a wide range of lab environments.
- Supports methods needing consistent flow precision and detector reliability for qualitative and quantitative analyses.

Instrument Application:

- Analysis of sugars, carbohydrates, and alcohols using the RID detector
- Quantification of UV-active compounds, such as pharmaceuticals or impurities, with the VWD
- Routine quality-control testing of raw materials and finished products
- Separation and profiling of small organic molecules in research or manufacturing settings

The Key Features:

- Stable, reliable HPLC performance
- Precise quaternary solvent delivery
- Controlled, accurate sample handling
- Flexible column temperature control
- UV and RI detection in one system
- Suited for routine daily analyses

Additional technical info:

- Brochure: Agilent 1260 Infinity ll LC System Brochure
- Manual: Agilent 1260 Infinity II Quaternary Pump Manual
- Manual: Agilent 1260 Infinity II Vialsampler Manual
- Manual: Agilent 1260 Infinity II Multicolumn Thermostat Manual
- Manual: Agilent 1260 Infinity II Variable Wavelength Detector Manual
- Manual: Agilent 1260 Infinity II Refractive Index Detector Manual
- Manual: Agilent 1200 Instant Pilot Manual

WHY DO CUSTOMERS MAKING CRITICAL LAB PURCHASES CHOOSE TO ORDER CHROMATOGRAPHY SYSTEMS FROM US?

They want to ensure that flow rate accuracy and solvent delivery stability were verified, preventing retention time drift, peak distortion, and irreproducible gradients.

They want confirmation that sampler leak testing was performed to prevent sample loss or air introduction into the injection path.

They want to ensure the column oven’s heating and cooling performance is tested, guaranteeing stable thermal control across analytical methods.

They want confirmation that wavelength accuracy was verified using a Holmium Oxide test, ensuring correct peak identification and spectral integrity.

They want confirmation that noise performance was tested, avoiding drifting baselines that can take hours to stabilize in poorly validated systems.

They want peace of mind knowing the system was tested module-by-module and as an integrated LC platform.

They want peace of mind, knowing their instrument is backed by a warranty from a company that can stand behind it.

Our Advantage

- We have experience servicing and repairing these instruments.
- This instrument goes through all the described service steps before shipping. see the questions section below
- We offer detailed photos and videos of the instruments you buy.
- We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
- We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
- Technical sales consultation available
- Remote installation and instrument operation training may be available.
- On-site installation may be available in the Boston Area (New England area)
- US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

Our Guarantee

- Warranty
- An extended warranty may be available
- You get after-sales technical support
- Customer references are available
- The instrument will be fully functional!
- Smooth process from the initial quote to order fulfillment

This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

PLEASE NOTE THAT THE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE

Q: Can I view this instrument in person at your facility or do a live Zoom demo before it is shipped to me?

A: We can accommodate both in-person or Zoom demos.

Q: What about the warranty?

A: The instrument comes fully warranted for both parts and labor.

Q: Do you offer installation, service, etc?

A: We offer remote installation and training packages to familiarize you with this instrument.

Q: What should I watch out for when buying a used/pre-owned instrument from another lab?

A: Ensure that the instrument is in good working condition with no damage or wear that could affect its performance. Ask for details about testing steps performed, video demo, and test screenshots.

Q: Do you have more than one of these instruments on hand?

A: We generally carry multiple instruments on hand, along with a variety of spare parts.

Q: This procurement is for a University or College. Are purchase orders with NET terms accepted?

A: We accept purchase orders from all major US and Canadian universities.

Q: How fast will you ship?

A: We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.

Q: Will my instrument be insured during shipping?

A: Absolutely! We insure all our shipments as long as we are the shipper. If a customer ships on their account, they handle insurance.

Q: How do you pack? Have you previously packed and shipped the fragile laboratory instruments?

A: We have extensive experience shipping high-end and complex laboratory instruments in the US and Internationally.

Q: Do you ship Internationally?

A: Yes! We have shipped to multiple continents and over 30 countries.

Q: How much will I save buying it pre-owned, and is it worth it?

A: When compared to buying a new instrument, you will save 60% or more and have plenty of funds left over for an additional instrument or two.