Beckman Coulter CytoFLEX LX U3-V5-B3-Y5-R3-IR2 Flow Cytometer (6)Lasers/(21)Colors/(23)Detectors in Walpole, MA, USA

- Good cosmetic condition
- Technical sales consultation available

Here is what you will receive:

- Beckman Coulter CytoFLEX LX Flow Cytometer Ref: C41917
- Desktop Computer Running CytExpert Software (with Monitor, Keyboard, and Mouse)
- CytoFLEX Plate Loader DW
- Power and Communication Cables.

Please note: You will need to purchase a CytExpert software License. CytExpert Software License Dongle is not included.

CytoFLEX LX Flow Cytometer Configuration: Default configuration per manufacturer website – we are unable to confirm without software

Optics:

- (6) Lasers, (21) Colors, (23) Detectors
- Laser Color Allocation (Laser/Channel Count): UV3-V5-B3-Y5-R3-IR2
- Forward Scatter Channel
- Side Scatter Channel
- 375 nm ultraviolet laser: 450/45, 525/40, 675/30
- 405nm violet laser: 450/45, 525/40, 610/20, 660/10, 763/43
- 488nm blue laser: 525/40, 610/20, 690/50
- 561nm yellow laser: 610/20, 763/43, 585/42, 675/30, 710/50
- 638nm red laser: 660/10, 712/25, 763/43
- 808 nm infrared laser: 840/20, 885/40

Fluidics:

- Sample input: single tube or DW plate loader
- Single Tube Loader: 5 mL (12 x 75 mm) polystyrene and polypropylene sample tubes 1.5 mL and 2 mL micro-centrifuge sample tubes
- Dead Volume: 10 uL
- Minimum Sample Volume:100 uL
- Plate Loader plate format: Flat/U/V bottom standard 96-well plate, 96-well deep well plate
- Dead Volume: 96-well deep well plate 20 uL; Flat bottom standard 96-well plate 20 uL; U/V bottom standard 96-well plate 10 uL
- Minimum Sample Volume: Standard 96-well Plate: 50 uL/well; Deep Well Plate: 200 uL/well
- Carryover: ≤1.0%

Performance:

- Signal acquisition speed: 30,000 particles/second with 23 parameters
- Sample flow rates: Fixed Flow Rates: 10 uL/min, 30 uL/min, 60 uL/min; Custom Flow Rates: 10 - 240 uL/min in 1 uL increments
- Throughput 10 sec acquisition without mixing or backflush: 96-well plate: <39 min.; DW 96-well plate: <40 min
- Throughput 10 sec acquisition w/ 5 sec mixing and 6 sec backflush: 96-well plate: <60 min.; DW 96-well plate: <69 min
- Sensitivity: <30 MESF for FITC, <10 MESF for PE, <25 MESF for APC
- Resolution: <300 nm particles (488 nm side scatter), rCV 3%
- Power Requirements: 100-240 VAC, 50/60 Hz, 300 VA, 5A
- Dimensions (D x W x H): 73.3 x 60.5 x 45.1 cm
- Weight: 84 kg

Additional Optics Configuration capability

The instrument has the capacity for 23 parameters, including 21 for fluorescence detection. The fully activated instrument includes three

fluorescent channels from the 355 nm (UV) laser, five from the 405 nm (Violet) laser, three from the 488 nm (Blue) laser, five from the 561 nm

(Yellow/Green) laser, three from the 638 nm (Red) laser, and two from the 808 nm (Infrared) laser. Instruments with as few as 14 fluorescent

channels activated are available with the ability to activate additional parameters as needed by purchasing an activation key.

Customization/Options: N/A

Testing performed by our in-house service team: Untested

SOLD AS IS / NO WARRANTY / NO RETURNS OR REFUNDS DUE TO LACK OF SOFTWARE AND INABILITY TO FULLY TEST.

- A visit from the manufacturer will be needed to configure the software license for the instrument.

Instrument Overview:

- Beckman Coulter CytoFLEX LX U3-V5-B3-Y5-R3-IR2 offers 6 lasers for high-parameter flow cytometry experiments.
- The system configures up to 21 fluorescence colors for detailed multi-color analysis of cell populations.
- Compact optical design reduces light loss and maximizes sensitivity for low-abundance markers.
- The CytoFLEX LX has 23 independent detectors with customizable filter sets for flexible panel design.
- Enhanced fluidics ensure stable flow rates and precise sample delivery for Beckman Coulter CytoFLEX LX.
- High-throughput capability supports large sample volumes while maintaining resolution and reproducibility.

Instrument Application:

- Immunophenotyping of complex cell populations
- Rare cell detection (e.g., stem cells, CTCs)
- Multiparametric cytokine profiling
- Cell cycle and apoptosis analysis

The Key Features:

- 6 laser excitation capability
- Detects 21 fluorescence colors
- 23 independent photomultiplier tubes
- High sensitivity, low-background optics
- Includes DW Plate Loader

Additional technical info:

- Datasheet: Beckman Coulter CytoFLEX LX Flow Cytometer Datasheet
- Brochure: Beckman Coulter CytoFLEX LX Flow Cytometer Brochure
- Manual: Beckman Coulter CytoFLEX LX Flow Cytometer Manual

Why do so many customers choose to order from us?

Our Advantage

- We have experience servicing and repairing these instruments.
- We offer detailed photos and videos of the instruments you buy.
- We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
- We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
- Technical sales consultation available
- US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

Our Guarantee

- Customer references are available
- Smooth process from the initial quote to order fulfillment

This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

*SM* Small Crate

225#

PLEASE NOTE THAT THE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE

Q: Can I view this instrument in person at your facility or do a live Zoom demo before it is shipped to me?

A: We can accommodate both in-person or Zoom demos.

Q: What about the warranty?

A: The instrument comes fully warranted for both parts and labor.

Q: Do you offer installation, service, etc?

A: We offer remote installation and training packages to familiarize you with this instrument.

Q: What should I watch out for when buying a used/pre-owned instrument from another lab?

A: Ensure that the instrument is in good working condition with no damage or wear that could affect its performance. Ask for details about testing steps performed, video demo, and test screenshots.

Q: Do you have more than one of these instruments on hand?

A: We generally carry multiple instruments on hand, along with a variety of spare parts.

Q: This procurement is for a University or College. Are purchase orders with NET terms accepted?

A: We accept purchase orders from all major US and Canadian universities.

Q: How fast will you ship?

A: We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.

Q: Will my instrument be insured during shipping?

A: Absolutely! We insure all our shipments as long as we are the shipper. If a customer ships on their account, they handle insurance.

Q: How do you pack? Have you previously packed and shipped the fragile laboratory instruments?

A: We have extensive experience shipping high-end and complex laboratory instruments in the US and Internationally.

Q: Do you ship Internationally?

A: Yes! We have shipped to multiple continents and over 30 countries.

Q: How much will I save buying it pre-owned, and is it worth it?

A: When compared to buying a new instrument, you will save 60% or more and have plenty of funds left over for an additional instrument or two.